User blogs

Hi all, 

We (a small team of software engineers and flow cytometrists) built a new tool for flow cytometry panel design and recently released online. It's called EasyPanel (link below).

It goes one step beyond what other online tools offer by actually suggesting specific optimized panels based on user's requests and requirements. It does so through an algorithm that Ranks all possible panels according to certain criteria such as systematically minimizing spectral overlap (spillover) between fluorochromes in the panel (among others). It could propose optimized panels of up to 19 fluorochromes.

Please give it a try and let me know what you think at

I need to find a tech who is knowledgeable about the CytoFlex S Flow Cytometer but i don't want to go to Beckman.   I am willing to pay for the knowledge if it is useable.   I would prefer someone who is on the west coast.  
stephen solender Jan 20 · Tags: looking
I need to find a tech who is knowledgeable about the CytoFlex S Flow Cytometer but i don't want to go to Beckman.   I am willing to pay for the knowledge if it is useable.   I would prefer someone who is on the west coast.  

Axle Informatics, LLC is currently seeking a Scientific Operator for Flow Cytometry Instruments for the National Institute of Health (NIH); Bethesda, Md. 



Scientific Operator for Flow Cytometry Instruments



To provide an outstanding scientific operator for flow cytometry instruments at the National Institutes of Health in Bethesda, MD.  The Flow Cytometry Facility in the Research Technologies Branch (RTB), in the Division of Intramural Research (DIR), National Institutes of Health.  The research agenda of the DIR focuses mostly on immunology and infectious disease research, and flow cytometry is central to many research projects.  The DIR supports approximately 120 principle investigators and 500 postdoctoral and clinical fellows. The Facility currently hosts six high speed cell sorters, five cell analyzers, and one imaging cytometer. Operations covers both BSL2 and BSL3 laboratories.


Responsibilities and Duties

  • Contribute to all aspects of facility operation, including flow cytometry analysis and cell sorting with added emphasis on independent operation of cell sorters inside both BSL2 and BSL-3 labs.
  • Perform daily Quality Control and maintenance.
  • Perform troubleshooting of Flow Cytometry equipment.
  • Provide support to researchers in operating analyzers.
  • Assist with user training and basic panel development.
  • Perform sample preparation and staining for flow cytometry analysis.
  • Assist in the preparation of SOPs and related documents for GLP work.
  • Comply with safety guidelines and requirements by following and ensuring strict safety procedures and safety checks.
  • Assist in Research and Development programs for new assays and technologies 
  • Qualifications

    • Positions require bachelor’s or master’s degree in Biology, Immunology, or related field.
  • Minimum five (5) years or more of demonstrated relevant experience in flow cytometry.
  • Experience in the operation of cell sorters is highly preferable.
  • Proficiency with basic laboratory techniques (sample preparation, cell culture) and flow cytometry methods such as immunophenotyping, cell cycle analysis, etc.
  • Experience in high parameter flow cytometry will be an advantage, but training can be provided where required.
  • Good understanding of laboratory biosafety standards.
  • Proficiency in the use of Microsoft Office including knowledge of computer systems and ability to work independently with data analysis programs
  • Ability to multi-task in a busy facility and to approach tasks and obstacles with a team-first mentality.
  • Development of new methods that extend the scope of flow cytometry at the facility will require excellent laboratory skills and a high level of desire to learn new skills

    For additional questions or concerns please contact Stefonie Kelly at or 202.421.2402.

    if you have a passion for Flow Cytometry and want to join a progressive company, give me a call. We are adding new sponsors and increasing our service offerings to existing sponsors.   Full time roles with benefits. Greg Inguagiato 904-831-5682

    Senior Scientist – Flow Cytometry

    Valencia, CA


    Job Summary

    Resource Solutions is working on behalf of our client, Q2 Solutions, the world’s second largest clinical laboratory services provider. Launched in 2015 through joint venture between Quintiles and Quest Diagnostics, Q2 Solutions is focused on helping biopharmaceutical, medical device and diagnostics customers improve human health through innovation that transforms science and data into actionable medical insights.



    • Assist with optimizing and improving flow cytometry operations
    • Perform routine technical troubleshooting of flow cytometry instruments and assays
    • Perform bench work and routine gating for operational support
    • Performs data analysis and reporting of flow cytometry clinical samples across a variety of cytometry assays.
    • Provides technical expertise, leadership and support for flow cytometry operations for assay/result troubleshooting.
    • Reviews and documents the results of technology transfers and process improvement validations. Collaborates with the Translational Sciences Laboratory where appropriate.
    • Investigate and resolve operational and quality issues within the flow cytometry department.
    • Write and review standard operating procedures and policies for the department.
    • Assist in the qualification and validation of new or repaired instruments.
    • Assists with training for flow cytometry staff for new and existing assays.
    • All responsibilities are essential job functions unless noted as nonessential (N).


    Required Knowledge, Skills and Abilities

    • A good understanding of equipment, software and processes of flow cytometry and the overall subject area.
    • Knowledge of clinical and/or research laboratory environment and quality control procedures.
    • Excellent Excel skills.
    • Ability to balance multiple priorities and work to short deadlines.
    • Ability to establish and maintain effective working relationships with co-workers, managers and clients.
    • Good oral communication skills.


    Minimum Required Education and Experience

    • Bachelors degree in chemical, physical, biological science, or clinical laboratory science
    • 5+ years of laboratory related experience with Flow Cytometry, experience in regulated clinical lab is preferred
    • CLIA and ASCP certification is highly preferred, not required


    About our Client

    With more than 9 million clinical laboratory tests managed annually, we are committed to providing our customers an innovative, progressive and responsive partner with the quality focus, global experience and deep medical expertise integral to drug, medical device and diagnostic development.

    We work collaboratively with our customers, business partners and colleagues to lead the industry and live our customer promise of providing Actionable Insights for Better Health™.


    Our commitment to innovation is how we’re building better clinical laboratory services for our customers’ – we call it “the lab of the future.” The lab of the future will help our customers develop drugs faster and more efficiently. Learn more about how we partner with our customers to build the lab of the future through: Tailored Solutions, Delivery Excellence and Shaping Outcomes.


    What we Offer

    ·         Major Medical, Vision, Dental, Disability

    ·         Annual bonus

    ·         401(k) with 100% vesting after 2 years

    ·         Tuition Reimbursement up to $6k annually

    ·         Generous paid time off and holiday leave

    ·         Other generous benefits


    EEO Minorities/Females/Protected Veterans/Disabled




    Dear All

    Does anyone know if there is a Euroflow users group?

    We are experiencing some issues with EQA material on a certain EQA program. The same issues are not present in our patient material.

    What EQA programs do other people use - especially on Euroflow?

    Any body having issues?


    South Africa

    Almero Du Pisani Nov 2 '18 · Tags: euroflow eqa
    we are going to distinguish NKcell population within PBMC through flow cytometry. Our approach is as follows:- 1. From the dot plot of FSC vs SSC we r gating CD-3 Cy5|| CD-56 FITC. 2. To isolate NK cell We are choosing the CD3-/CD56+ cells. 3. As we wish to detect the NK cell dim/bright population, we then gate CD56 FITC||CD16PE population. 4.At this stage we have isolated NK population and also differentiated NK dim/bright cells also. 5. Next we are going to observe CD-328 PE along with CD56 FITC dim/bright. 6. Similarly CD-329 PE will be observed. IMPORTANT NOTE: Our constraints:- 1.we can not perform 6 color facs. 2. We have isotype control having 3 fluorophores namely FITC, PE, Cy5 which can't be altered due to budget constraint. 3.we have access to a BDFacscalibur having 4color only namely FL1,FL2,FL3,FL4. We prefer to perform 2color face. 4. Due to budget constraint , we will set the CD3 and CD16 gates first and then further just gate the PBMC through the previously selected gate. Then we will just tag the PBMC with CD56 & CD328/CD329 is it okay? Waiting for your kind reply.

    We have available a unique cell-tracing product to the pharmaceutical industry that enables 10 × higher visibility and dramatically longer tracing of cells than any other product on the market today.  If interested please respond.

    Chalonda Handy Feb 20 '15

    Silicon Microchip Capable of High-Frequency Fluidic Valving at Heart of Technology
    Jim Linton, Ph.D. Shane W. Oram, Ph.D.

    The purification of cellular populations and individual cells is pivotal for the reliable characterization of gene expression, many aspects of life science research, and the development of cellular therapies. Cell sorting routinely involves fluorescence-based separation through flow cytometry, which has proven superior to crude techniques such as differential sedimentation. Through this process, large numbers of cells are rapidly analyzed for specific fluorescence signatures. Traditionally, cell samples are parsed in charged aerosol droplets, which are electrostatically sorted, enabling purification at thousands of cells per second at purities often greater than 90%.

    This technology enables exceptional specificity using multiple fluorescent signatures (e.g., cell surface labels, cell size, and granularity).

    However, the contributions of current flow-sorting platforms are balanced against significant limitations, including: high processing pressures that can result in loss of function and/or cell death; sample processing speeds/volumes that make processing clinical-scale samples (>500 million cells) unfeasible; a high degree of technical expertise needed to manage device complexity; increased risk of sample contamination through the use of open systems; and user safety concerns when processing aerosolized patient samples.

    These limitations, plus the high unit and sample processing costs, must be overcome to enable further clinical application and commercialization.

    To address these issues, Owl Biotech developed a fully closed cell-sorting system. This microchip-based technology employs closed fluid path cartridges with aseptic ports that permit the straightforward administration and collection of cell samples. At the heart of the cartridge is a patented microchip capable of very high-frequency fluidic valving (Figure 1).

    Propelled by modest positive pressure, typically less than 0.2 atmospheres, cells pass through microchannels where laser-directed fluorescent signals are detected with photo-multiplier tubes. Upon identification of a positive target cell, the microchip valve opens, redirecting the cell to a collection chamber. Both positive and negative selected cells can then be retrieved from the cartridge and used for any number of downstream applications.

    Improving Valve Speed Click Image To Enlarge +
    Figure 2. Mechanism of microchip-based sorting: Labeled cell samples enter the chip from the input sample, as the cells approach the sort area each cell is analyzed. When a selected cell is identified a magnetic pulse opens and closes the valve and the cell is redirected to a collection chamber. An integrated single-crystal silicon spring returns the valve to its original position, and undesired cells are allowed to flow through.

    One key design goal of all cell sorters is to maximize the speed at which the device can segment a stream of cells. In the case of Owl’s microchip-based technology, a fluidic valve determines the rate at which cells are isolated. Valve speed in a fluid microenvironment is in turn controlled by several factors, including the acceleration and magnitude of the opening and closing forces, and the inertia of the valve and the fluid surrounding it.

    In the case of Owl technology, valve speed is controlled by its engineered magnetic properties and a powerful return-spring force, which serves to close the valve (Figure 2). Careful modeling and empirical testing has led to a design that allows μsec opening times, a user-selected sort collection delay, and μsec closing times.

    A typical total cycle of around 50 μsec allows separation rates similar to a traditional droplet sorter, although with microchip-based sorting no aerosols or droplets are used.

    Highly Viable Sorted Cells

    Sorted cells are typically used for molecular analysis or sample preparation, for example, cellular expansion for research or therapeutic purposes. In such cases, those cells need to be in a healthy metabolic state ideally retaining their complete array of functional capabilities. Current flow cytometric sorting has distinct challenges in that respect due to technical requirements such as high pressure, extended shear rate, severe decompression upon aerosolization, and impact trauma during cell collection. Often, these factors result in cell isolates with compromised function and/or viability.

    Using Owl’s microchip-based technology, the pressure applied to cells is minimal and the trauma associated with droplet sorting is removed, resulting in high cell viability. In addition, a wide variety of cell types have been sorted using the Owl technology, all with a high retention of cell functionality. For example, antigen-primed T cells have been shown to retain their cancer-specific cytotoxic capabilities in chromium-release assays.

    Microchip-Based Sorting Click Image To Enlarge +
    Figure 3. Sorting target cells from dilute whole blood or PBMCs: Pre-sort fraction of diluted whole blood with CD4+ cells (A) Post-sort fraction showing enrichment of CD-4 positive cells (B) Pre-sort fraction of the spiked MART-1-specific T-cell clone into PBMCs (C) Post-sort fraction showing enrichment of MART-1 positive antigen specific T cells (D).

    While the utility of microchip-based cell separation is being tested in many different applications, current studies show its utility in processing clinical samples for diagnostic and therapeutic applications. For these purposes it is best to process the samples with as little manipulation as possible.

    Here, CD4+ cells were sorted from a diluted whole blood preparation by adding a fluorescent CD4 antibody marker to 8 mL PBS and 2 mL of whole blood. Results show that while the presorted fraction contained less than 0.01% CD4 positive cells, the sorted fraction contained more than 91% CD4 positive cells (Figures 3A and B). The effective purification was close to 10,000-fold in a single-step process and with a simple no-lyse, no-wash sample preparation.

    Investigations have also been done to study the ability of antigen-specific T cells from a patient’s own blood to recognize tumor cells and trigger an immune system response. To explore the feasibility of this clinically important strategy, cells from a MART-1-specific T cell clone were spiked into a patient’s peripheral blood mononuclear cells (PBMCs), stained with a PE-conjugated tetramer loaded with a MART-1 peptide, and then sorted using the Owl’s microchip-based sorting technology.

    Results show tetramer-positive cells were enriched from less than 1% to greater than 95% (Figures 3C and D). Importantly, sorted cells maintained their ability to kill MART-1+ tumor cells and to proliferate in vitro.


    Acceleration of research for effective cancer and cellular therapies has increased the need for cell-sorting technologies that are safe, efficient, and easy to use. Owl’s cartridge-based closed system has effectively demonstrated its capability to sort a wide variety of cells at high speeds without impacting cell viability. This platform offers the additional advantage of protecting sample integrity while permitting quick sample-to-sample changeover—a feature that avoids the inter-sample cleaning and validation required for traditional cell sorting.

    That this technology does not utilize sheath fluids opens the future potential for sequential sorting of large numbers of samples within a short timeframe, making practical clinical applications a possibility. In addition, combining the underlying microchip technology with other cell isolation techniques such as magnetic beads has the potential to empower processing of samples greater than 1 billion cells.

    Jim Linton, Ph.D. (, is chief business officer at Owl biomedical, and Shane W. Oram, Ph.D. (, is global marketing manager—cell analysis at Miltenyi Biotech.

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