Non Specific Binding of Antibodies in Immunoassays
Flow Cytometry Network
How to Overcome all of your Problems with Secondary Antibodies
The
latest Innova Biosciences webinar focuses on how to overcome the
problems of using secondary antibodies. For instance, the use of
secondary antibodies:
• Requires a series of incubations and wash
steps that are both tedious and time consuming. It is amazing how many
times people state how much they hate those wash steps!
• Can
often be a source of non-specific staining within experiments which make
data interpretation difficult or even impossible.
• Multi-colour analysis often results in cross species re-activity.
Secondary
antibodies are generally used either because there are no directly
labeled primary antibodies or to increase sensitivity. In this seminar,
we will review:
• How labeling of your own antibodies overcomes the need for secondary antibodies.
•
How easy it really is to label an antibody using Innova's 30 seconds
hands-on antibody labeling kits and design your own unique research
tools.
• Application data such as flow cytometry and western blotting generated using directly labeled antibodies
• And question the hypothesis of secondary vs. primary labeled antibodies.
The Wall