Flow Cytometry, Fluorochromes, Flow Cytometry Fluorochromes
This comprehensive table of Fluorochromes, Fluorescent Proteins, Fluorescent Probes, and Bio-Indicators for Flow Cytometry has been consolidated from various sources online. The listing is in alphabetical order that makes it easy to find. You could also use "find" option (control+F in PC or command+F in Mac) to locate an entry faster. You could suggest us to add an entry if you do not see something you are looking for.
Fluorochrome/Fluorescent Protein/Probe/Indicator | Ex (nm) | Em (nm) | MW | Oligomeric State | Mol. Ext. Coeff (e) | Quantum Yield (QY) | Brightness (exQY/100) | Comments/Use |
---|---|---|---|---|---|---|---|---|
7-aminoactinomycin D (7-AAD) | 546 | 647 | CG-selective | |||||
AcGFP1 | 484 | 510 | 27 | 0.82 | 27 | |||
Acridine Orange | 503 | 530/640 | DNA/RNA | |||||
Alexa Fluor 350 | 343 | 442 | 410 | 19000 | ||||
Alexa Fluor 405 | 401 | 421 | 1028 | 34000 | ||||
Alexa Fluor 430 | 434 | 540 | 702 | 16000 | ||||
Alexa Fluor 488 | 499 | 519 | 643 | 0.92 | ||||
Alexa Fluor 500 | 503 | 525 | 700 | 71000 | ||||
Alexa Fluor 514 | 517 | 542 | 714 | 80000 | ||||
Alexa Fluor 532 | 530 | 555 | 724 | 0.61 | ||||
Alexa Fluor 546 | 561 | 572 | 1079 | 0.79 | ||||
Alexa Fluor 555 | 553 | 568 | 1250 | 150000 | 0.1 | 150 | ||
Alexa Fluor 568 | 579 | 603 | 792 | 0.69 | ||||
Alexa Fluor 594 | 590 | 617 | 820 | 73000 | 0.64 | 467.2 | ||
Alexa Fluor 610 | 610 | 629 | 1285 | 138000 | ||||
Alexa Fluor 632 | 632 | 648 | 1200 | 100000 | ||||
Alexa Fluor 647 | 652 | 668 | 1300 | 0.33 | ||||
Alexa Fluor 660 | 663 | 690 | 1100 | 132000 | 0.37 | 488.4 | ||
Alexa Fluor 680 | 680 | 702 | 1150 | |||||
Alexa Fluor 700 | 696 | 719 | 1400 | 192000 | 0.25 | 480 | ||
Alexa Fluor 750 | 752 | 776 | 1300 | |||||
Alexa Fluor 790 | 782 | 804 | 1750 | |||||
Allophycocyanin (APC) | 650 | 660 | 104 | |||||
AmCyan | 458 | 489 | 40000 | 0.24 | 96 | |||
AmCyan1 | 458 | 489 | tetramer | 0.75 | 29 | |||
Aminocoumarin | 350 | 445 | 330 | Succinimidyl ester | ||||
APC Cy5.5 | 650 | 690 | ||||||
APC Cy7 | 650 | 774 | ||||||
APC-H7 | 650 | 785 | ||||||
APC/Alexa Fluor 750 | 650 | 774 | 240000 | 0.12 | 288 | |||
AsRed2 | 576 | 592 | 110 | tetramer | 0.21 | 13 | ||
Azami-Green | 492 | 505 | tetramer | 0.74 | 41 | monomer available | ||
Azurite | 383 | 447 | monomer | 0.55 | 14 | |||
B ODIPY FL C5-ceramide | 488 | 500 | Golgi Labeling | |||||
BCECF-AM | 403 | 528 | pH | |||||
Bis-oxonol DiBAC2(3) | 493 | 560 | Plasma membrane potential | |||||
BODIPY-FL | 503 | 512 | ||||||
Calcein | 496 | 517 | 623 | pH > 5 | ||||
Calcein AM | 494 | 517 | Mitochondrial Transition pore | |||||
Caroxy-H2DCFDA | 504 | 529 | H2O2 | |||||
Cascade Blue | 398 | 420 | 596 | 29000 | 0.5 | 145 | ||
Cascade Yellow | 402 | 545 | 25000 | 0.56 | 140 | |||
Cell Tracker Green | 492 | 517 | Cell tracking | |||||
Cerulean | 433 | 475 | Monomer | 0.62 | 27 | |||
CFSE | 494 | 514 | Cell Proliferation | |||||
Chromomycin A3 | 445 | 575 | CG-selective | |||||
CM-H2DCFDA | 495 | 529 | H2O2 | |||||
Cy2 | 489 | 506 | 714 | 0.12 | ||||
Cy3 | 514, 547 | 566 | 767 | 150000 | 0.15 | 225 | ||
Cy3.5 | 581 | 594;(640) | 1102 | 0.15 | ||||
Cy3B | 558 | 572;(620) | 658 | 0.67 | ||||
Cy5 | (625);650 | 670 | 792 | 0.28 | ||||
Cy5.5 | 675 | 694 | 1128 | 0.23 | ||||
Cy7 | 743 | 767 | 818 | 0.28 | ||||
CyPet | 435 | 477 | Monomer | 0.51 | 18 | |||
DAF-FM | 495 | 515 | Nitric Oxide | |||||
DAF-FM diacetate | 495 | 515 | Nitric Oxide | |||||
DAPI | 359 | 461 | 27000 | 0.58 | 156.6 | AT-selective | ||
DCFH (2'7'Dichorodihydrofluorescein) | 505 | 535 | 529 | Oxidized form | ||||
DHR | 505 | 534 | 346 | Dihydrorhodamine 123, oxidized form, | ||||
Dihydrocalcein AM | 494 | 517 | H2O2 | |||||
Dihydrorhoadamine | 488 | 525 | H2O2 | |||||
Dihydrothidium | 518 | 605 | Superoxide Anion | |||||
DiLC1(5) | 638 | 658 | Mitochondrial membrane potential | |||||
DiOC6(3) | 488 | 500 | Mitochondrial membrane potential | |||||
DiOC7(3) | 488 | 500 | Mitochondrial membrane potential | |||||
dKeima-Red | 440 | 616 | dimer | 0.31 | 8 | |||
DRAQ5 | 647 | 681697 | 413 | usable excitation down to 488 | ||||
Dronpa-Green | 503 | 518 | 0.85 | 81 | photoswitchable | |||
DsRed monomer | 557 | 592 | 28 | monomer | 0.1 | 3.5 | ||
DsRed tetramer | 554 | 591 | tetramer | |||||
DsRed-Express2 | 554 | 591 | 0.42 | 15 | ||||
DsRed2 ("RFP") | 563 | 582 | 110 | 0.55 | 24 | |||
dTomato | 554 | 581 | dimer | |||||
DyLight 350 | 353 | 432 | ||||||
DyLight 405 | 400 | 420 | ||||||
DyLight 488 | 493 | 518 | ||||||
DyLight 549 | 562 | 576 | ||||||
DyLight 594 | 593 | 618 | ||||||
DyLight 633 | 638 | 658 | ||||||
DyLight 649 | 654 | 673 | ||||||
DyLight 680 | 692 | 712 | ||||||
DyLight 750 | 752 | 778 | ||||||
DyLight 800 | 777 | 794 | ||||||
E.coli BioParticles AF488 | 495 | 519 | Phagocytosis | |||||
E2-Crimson | 611 | 646 | tetramer | |||||
E2-Crimson | 611 | 646 | 0.23 | 29 | ||||
E2-Orange | 540 | 561 | tetramer | |||||
EBFP | 380 | 440 | monomer | 0.18 | 9 | |||
EBFP2 | 383 | 448 | monomer | 0.56 | 18 | |||
ECFP | 434 | 477 | Monomer | 26000 | 0.4 | 104 | ||
eFluor 490 NC | 470 | 490 | 59500 | |||||
eFluor 525 NC | 505 | 525 | 57800 | |||||
eFluor 565 NC | 550 | 565 | 105000 | |||||
eFluor 585 NC | 570 | 585 | 157000 | |||||
eFluor 605 NC | 590 | 605 | 253000 | |||||
eFluor 625 NC | 610 | 625 | 458000 | |||||
eFluor 650 NC | 640 | 650 | 1110000 | |||||
eFluor 690 NC | 660 | 690 | 3240000 | |||||
EGFP | 488 | 507 | 26 | monomer | 0.6 | 34 | weak dimer | |
EGFP* | 489 | 508 | 55000 | 0.6 | 330 | |||
Emerald | 487 | 509 | monomer | 0.68 | 39 | weak dimer | ||
eqFP650 | 592 | 650 | dimer | |||||
eqFP670 | 605 | 670 | dimer | |||||
ER-Tracker Blue-White DPX | 375 | 520 | ER Labelling | |||||
Ethidium Bromide | 493 | 620 | 394 | |||||
Express2 | 574 | 596 | monomer | |||||
EYFP | 514 | 527 | 26 | monomer | 0.61 | 51 | weak dimer | |
Fc OxyBurst Green | 488 | 525 | Superoxide Anion | |||||
Fc OxyBurst Green 123 AM | 488 | 525 | H2O2 | |||||
FITC | 493 | 525 | 78000 | 0.5 | 390 | |||
Fluo-3 | 506 | 526 | 855 | AM ester. pH > 6 | ||||
Fluo-4 | 494 | 516 | Calcium Signalling | |||||
Fluorescein | 495 | 519 | 389 | FITC; pH sensitive | ||||
FluorX | 494 | 520 | 587 | |||||
Fura-2 | 340-380 | 510 | Ratiometric Calcium signalling | |||||
Fura-Red | 458 | 597 | Ratiometric Calcium signalling | |||||
GFPuv | 385 | 508 | ||||||
H2DCFDA | 495 | 529 | H2O2 | |||||
HcRed1 | 588 | 618 | 52 | dimer | 0.03 | 0.6 | ||
Hoechst Blue (33258) | 352 | 455 | 624 | 40000 | 0.59 | 236 | AT-selective | |
Hoechst Red (33342) | 350 | 650 | 616 | AT-selective | ||||
Hydroxycoumarin | 325 | 386 | 331 | Succinimidyl ester | ||||
HyPer | 420/500 | 516 | Hydrogen peroxide sensor | |||||
Indo-1 | 360 | 400-500 | 1010 | Ratiometric Calcium signalling | ||||
Indo-1 Blue (Low Ca2+) | 350 | 475 | ||||||
Indo-1 Violet (High Ca2+) | 330 | 405 | 33000 | 0.56 | 184.8 | |||
iRFP | 690 | 713 | dimer | |||||
J-Red | 584 | 610 | dimer | 0.2 | 8.8 | |||
JC-1 | 514 | 529-575 | Mitochondrial membrane potential | |||||
JC-9 | 460-500 | 526-650 | Mitochondrial membrane potential | |||||
Katushka (TurboFP635) | 588 | 635 | 26 | dimer | 0.34 | 22 | ||
Katushka2 | 588 | 635 | dimer | |||||
Kusabira-Orange | 548 | 559 | monomer | 0.6 | 31 | |||
LDS 751 | 543;590 | 712;607 | 472 | DNA (543ex/712em), RNA (590ex/607em) | ||||
Lissamine Rhodamine B | 570 | 590 | ||||||
Live/Dead Aqua | 367 | 526 | ||||||
Live/Dead Blue | 350 | 450 | ||||||
Live/Dead Far Red | 650 | 665 | ||||||
Live/Dead Green | 495 | 520 | ||||||
Live/Dead Red | 595 | 615 | ||||||
Live/Dead Violet | 416 | 451 | ||||||
Live/Dead Yellow | 400 | 575 | ||||||
LSSmKate2 | 460 | 605 | monomer | |||||
Lucifer yellow | 425 | 528 | ||||||
Lucifer Yellow CH | 428 | 536 | 24200 | 0.21 | 50.82 | |||
Lyso Tracker Blue | 372 | 422 | Lysosome labelling | |||||
Lyso Tracker Green | 577 | 590 | Lysosome labelling | |||||
Lyso Tracker Red | 577 | 590 | Lysosome labelling | |||||
mAmertrine | 406 | 526 | monomer | |||||
Marina Blue | 362 | 459 | 18700 | 0.89 | 166.43 | |||
mBanana | 540 | 553 | monomer | 6000 | 0.7 | 42 | ||
mCFP | 433 | 475 | monomer | 0.4 | 13 | |||
mCherry | 587 | 610 | monomer | 72000 | 0.22 | 158.4 | ||
mCitrine | 516 | 529 | monomer | 0.76 | 59 | |||
Methoxycoumarin | 360 | 410 | 317 | Succinimidyl ester | ||||
mHoneyDew | 487 | 537 | 17000 | 0.12 | 20.4 | |||
Midoriishi-Cyan | 472 | 495 | dimer | 0.9 | 25 | |||
Mithramycin | 445 | 575 | ||||||
Mito Tracker Deep Red | 633 | 660 | Mitochondrial membrane potential | |||||
Mito Tracker Green | 490 | 516 | Mitochondrial labelling | |||||
Mito Tracker Orange | 554 | 575 | Mitochondrial labelling | |||||
Mito Tracker Red | 579 | 599 | Mitochondrial membrane potential | |||||
MitoFluor Green | 488 | 530 | Mitochondrial labelling | |||||
mKate (TagFP635) | 588 | 635 | monomer | 0.3 | 15 | |||
mKate2 | 588 | 633 | 26 | monomer | 0.4 | 25 | ||
mKeima | 440 | 620 | dimer | |||||
mKeima-Red | 440 | 620 | monomer | 0.24 | 3 | |||
mKO | 548 | 559 | monomer | 0.6 | 31 | |||
mKOk | 551 | 563 | monomer | |||||
mNeptune | 600 | 650 | monomer | 0.2 | 13 | |||
Monochlorobimane | 380 | 461 | 226 | Glutathione Redox Potential | ||||
mOrange | 546 | 562 | monomer | 71000 | 0.69 | 489.9 | ||
mOrange2 | 549 | 565 | monomer | 0.6 | 35 | |||
mPlum | 589 | 649 | 41000 | 0.1 | 41 | |||
mRaspberry | 597 | 624 | 86000 | 0.15 | 129 | faster photobleach than mPlum | ||
mRFP1 | 584 | 607 | 30 | monomer | 0.25 | |||
mStrawberry | 574 | 596 | monomer | 90000 | 0.29 | 261 | ||
mTangerine | 568 | 585 | 38000 | 0.3 | 114 | |||
mTarquoise | 434 | 474 | Monomer | |||||
mTFP1 | 462 | 492 | Monomer | |||||
mTFP1 (Teal) | 462 | 492 | dimer | 0.85 | 54 | |||
NBD | 466 | 539 | 294 | NBD-X | ||||
Oregon Green 488 | 496 | 516 | 76000 | 0.9 | 684 | |||
OxyBurst Green H2DCFDA | 488 | 525 | Superoxide Anion | |||||
OxyBurst Green H2HFF BSA | 488 | 525 | Superoxide Anion | |||||
Pacific Blue | 403 | 455 | 406 | 29500 | 0.78 | 230.1 | Maleimide | |
Pacific Orange | 403 | 551 | ||||||
PE (R-Phycoerythrin) | 480;565 | 578 | 240 | |||||
PE Cy5 | 496565 | 670 | ||||||
PE Cy5.5 | 496565 | 690 | ||||||
PE Cy7 | 496565 | 774 | ||||||
PE Texas Red | 496;565 | 613 | 116000 | |||||
PE-Cy5 conjugates | 480;565;650 | 670 | aka Cychrome, R670, Tri-Color, Quantum Red | |||||
PE-Cy7 conjugates | 480;565;743 | 767 | ||||||
PerCP | 482 | 690 | 320000 | |||||
PerCP (Peridinin chlorphyll protein) | 490 | 675 | ||||||
PerCP Cy5.5 | 535 | 620 | ||||||
PhiYFP | 525 | 537 | 26.8 | weak dimer | 0.4 | 52 | ||
PhiYFP-m | 525 | 537 | 26.8 | monomer | 0.39 | 48 | ||
Propidium Iodide (PI) | 536 | 617 | 668.4 | 360000 | ||||
Qdot 525 | 350, 405 | 525 | 360000 | |||||
Qdot 545 | 350, 405 | 545 | ||||||
Qdot 565 | 350, 405 | 565 | 1100000 | |||||
Qdot 585 | 350, 405 | 585 | 2200000 | |||||
Qdot 605 | 350, 405 | 605 | 2800000 | |||||
Qdot 655 | 350, 405 | 655 | 5700000 | |||||
Qdot 705 | 350, 405 | 705 | 8300000 | |||||
Qdot 800 | 350, 405 | 800 | 8000000 | |||||
Red 613 | 480;565 | 613 | PE-Texas Red | |||||
RFP Tomato | 554 | 581 | 69000 | 0.69 | 476.1 | |||
Rhod-2 | 530 | 575 | Organelle calcium signalling | |||||
S65A | 471 | 504 | ||||||
S65C | 479 | 507 | ||||||
S65L | 484 | 510 | ||||||
S65T | 488 | 511 | ||||||
SeTau-380-NHS | 270 | 480 | 472 | 23800 | 0.56 | 133 | Reactive dye | |
Seta-375-NHS | 372 | 476 | 514 | 24500 | 0.86 | 211 | Reactive dye | |
SeTau-405-Maleimide | 405 | 518 | 574 | 13800 | 0.51 | 70 | Reactive dye | |
SeTau-405-NHS | 405 | 518 | 549 | 13800 | 0.8 | 110 | Reactive dye | |
SeTau-405-Azide | 405 | 518 | 534 | 13800 | 0.8 | 110 | Reactive dye | |
SeTau-425-NHS | 425 | 545 | 543 | 4200 | 0.39 | 16 | Reactive dye | |
Seta-PerCP-680 | 481 | 676 | 36 k | 380000 | FC-tandem | |||
Seta-R-PE-670 | 566 | 671 | 246 k | 2090000 | FC-tandem | |||
Seta-555-NHS | 556 | 571 | 986 | 155000 | 0.13 | 202 | Reactive dye (IgG, D/P=1) | |
Seta-580-NHS | 578 | 602 | 632 | 90000 | 0.55 | 495 | Reactive dye | |
Seta-633-di-NHS | 639 | 650 | 1454 | 192000 | 0.33 | 634 | Reactive dye (IgG, D/P=1) | |
SeTau-647-Maleimide | 648 | 692 | 1873 | 200000 | 0.45 | 900 | Reactive dye | |
SeTau-647-NHS | 649 | 695 | 1846 | 200000 | 0.61 | 1220 | Reactive dye | |
SeTau-647-di-NHS | 650 | 694 | 1752 | 200000 | 0.65 | 1300 | Reactive dye | |
Seta-650-NHS | 654 | 673 | 1271 | 200000 | 0.37 | 740 | Reactive dye (IgG, D/P=1) | |
Seta-650-Azide | 651 | 671 | 969 | 200000 | 0.28 | 560 | Reactive dye | |
Seta-APC-780 | 651 | 780 | 111 k | 1000000 | FC-tandem | |||
Seta-650-Maleimide | 652 | 672 | 1296 | 200000 | 0.28 | 560 | Reactive dye | |
Seta-650-DBCO | 653 | 674 | 1432 | 200000 | 0.28 | 560 | Reactive dye | |
Seta-660-di-NHS | 665 | 675 | 1973 | 220000 | 0.49 | 660 | Reactive dye (IgG, D/P=1) | |
Seta-670-Maleimide | 667 | 686 | 1250 | 180000 | 0.07 | 126 | Reactive dye | |
Seta-680-NHS | 680 | 701 | 670 | 200000 | 0.3 | 600 | Reactive dye (IgG, D/P=1) | |
Seta-700-NHS | 687 | 703 | 1052 | 177000 | 0.06 | 106 | Reactive dye | |
Seta-700-di-NHS | 696 | 713 | 1287 | 188000 | 0.23 | 432 | Reactive dye (IgG, D/P=1) | |
Seta-750-NHS | 756 | 783 | 1297 | 230000 | 0.17 | 391 | Reactive dye (IgG, D/P=1) | |
Seta-780-NHS | 777 | 807 | 1232 | 215000 | 0.01 | 22 | Reactive dye | |
TruRed | 490675 | 695 | PerCP-Cy5.5 conjugate | |||||
TurboFP602 | 574 | 602 | 26 k | dimer | 0.35 | 26 | ||
TurboFP635 | 588 | 635 | 26 k | dimer | 0.34 | 22 | ||
TurboGFP | 482 | 502 | 26 k | dimer | 0.53 | 37 | ||
TurboRFP | 553 | 574 | 26 k | dimer | 0.67 | 62 | ||
TurboYFP | 525 | 538 | 26 | dimer | 0.53 | 56 | ||
Venus | 515 | 528 | monomer | 0.57 | 53 | weak dimer | ||
Vybrant CycleDye Violet | 370 | 435 | DNA Live cells | |||||
Wild Type GFP | 396/475 | 508 | 26 | 0.77 | 16 | |||
X-Rhodamine | 570 | 576 | 548 | XRITC | ||||
Y66F | 360 | 508 | ||||||
Y66H | 360 | 442 | ||||||
Y66W | 436 | 485 | ||||||
YOSeta-1 | 492 | 509 | 1083 | 98800 | 0.52 | 514 | DNA Probe | |
YOYO-1 | 491 | 509 | ||||||
YPet | 517 | 530 | monomer | 0.77 | 80 | weak dimer | ||
ZsGreen1 | 493 | 505 | 105 | tetramer | 0.91 | 40 | ||
ZsYellow1 | 529 | 539 | tetramer | 0.65 | 13 | |||
Zymosan A BioParticles AF488 | 495 | 519 | Opsonization |