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North Carolina State University - College of Veterinary Medicine


The Flow Cytometry and Cell Sorting Core facility is located in the College of Veterinary Medicine. The cell sorting unit is used for a wide variety of applications including sorting immune cells, bacterial cells, reproductive cells, and can also be used for separation of pharmaceutical delivery particles. The flow cytometry units are utilized for analysis of mammalian cells, avian (bird) cells, and other biological specimens. 

 

North Carolina State University’s College of Veterinary Medicine (CVM) is a dynamic community whose members are dedicated to preparing veterinarians and veterinarian scientists while advancing animal and human health from the cellular level through entire ecosystems. 

 

The College has gained international recognition on the strength of its teaching, research, engagement, and patient care efforts and is ranked 3rd among the nation’s 28 colleges of veterinary medicine in the current U.S. News & World Report survey.


The college is committed to practicing inclusivity, making community a priority and promoting innovation. We embrace effective leadership and we champion personal and professional growth. 


Essential Job Duties: Management of the Flow Cytometry and Cell Sorting Facility located in the College of Veterinary Medicine. The Manager will oversee all technical aspects and day-to-day management of this facility. Clients may include investigators in addition to those from the NCSU and College of Veterinary Medicine hospital, surrounding universities, and industry. The cell sorting unit is used for a wide variety of applications including sorting immune cells, bacterial cells, reproductive cells, and can also be used for separation of pharmaceutical delivery particles. The flow cytometry units are utilized for analysis of mammalian cells, avian (bird) cells, and other biological specimens.


The manager also supervises and monitors management of three other college core pieces of equipment: NanoString, XRad Navigator, and Sea Horse.  The manager will report results, maintain billing, and answer questions from submitting researchers. 


Additional duties will include interfacing with clients of the facility on research design and execution and organizing quarterly educational opportunities. The Manager will also be expected to assist in teaching/training of graduate students and faculty on the use of the Flow Cytometric analyzers. 


Apply Online Here

Med-Immunology - Pennsylvania-Pittsburgh - (21006827)

The incumbent will function as a Flow Cytometry Technologist I in a Unified Flow Cytometry Core Facility which equipped with 3 high speed sorters, 5 analyzers, an imaging cytometer and 2 innovative 48 channel analyzers. This individual will have primary responsibility for overseeing advanced flow cytometry analyzers, for maintenance and operation of instrumentation located across multiple physical sites. Additional responsibilities involve cell sorting and assisting investigators to run their own samples with the supervision of the Operational Director of Unified Core Facility. Other duties include independently performing daily and monthly instrument maintenance, maintaining laboratory records, ordering supplies. The percentage of the time is about 80% on the analyzer related activities including instrument operation, oversight and user education, and panel design, and 20% on the cell sorter related activities including hands-on sorting and also records management. The candidate will have opportunities to develop educational workshops, and learn advanced computational flow cytometry approaches. This position reports to the Operational Director of Unified Core Facility. The applicant will have a unique opportunity to contribute to the vision of a core that serves over 300 investigators with >$150 M in NIH grants, and is poised for additional growth. The applicant should be highly motivated, detail-oriented and able to prioritize multiple tasks. Sound judgment, strong analytical skills and exceptional interpersonal skills are a must.

A Bachelor's degree in biology or related field is required, laboratory experience or flow cytometry experience is a plus. Equivalent Relevant Work Experience may be substituted for degree requirement and vice versa.

The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets

Assignment Category Full-time regular
Job Classification Staff.Research.III Non-Exempt
Campus Pittsburgh
Minimum Education Level Required Baccalaureate
Minimum Experience Level Required 2-3 years experience
Work Schedule Monday - Friday, 8:30 a.m. - 5:00 p.m.
Hiring Range TBD based on qualifications
Relocation Offered No
Visa Sponsorship Provided No
Background Check For position finalists, employment with the University will require successful completion of a background check
Child Protection Clearances Not Applicable

Apply Here

PI153686496

Hi all, 

We (a small team of software engineers and flow cytometrists) built a new tool for flow cytometry panel design and recently released online. It's called EasyPanel (link below).

https://flow-cytometry.net/

It goes one step beyond what other online tools offer by actually suggesting specific optimized panels based on user's requests and requirements. It does so through an algorithm that Ranks all possible panels according to certain criteria such as systematically minimizing spectral overlap (spillover) between fluorochromes in the panel (among others). It could propose optimized panels of up to 19 fluorochromes.

Please give it a try and let me know what you think at omar_zid@flow-cytometry.net
Thanks!

I need to find a tech who is knowledgeable about the CytoFlex S Flow Cytometer but i don't want to go to Beckman.   I am willing to pay for the knowledge if it is useable.   I would prefer someone who is on the west coast.   Stephenmishka90025@yahoo.com  
stephen solender Jan 20 '21 · Tags: looking
I need to find a tech who is knowledgeable about the CytoFlex S Flow Cytometer but i don't want to go to Beckman.   I am willing to pay for the knowledge if it is useable.   I would prefer someone who is on the west coast.   Stephenmishka90025@yahoo.com  

Axle Informatics, LLC is currently seeking a Scientific Operator for Flow Cytometry Instruments for the National Institute of Health (NIH); Bethesda, Md. 

 


Overview

Scientific Operator for Flow Cytometry Instruments

 

Purpose

To provide an outstanding scientific operator for flow cytometry instruments at the National Institutes of Health in Bethesda, MD.  The Flow Cytometry Facility in the Research Technologies Branch (RTB), in the Division of Intramural Research (DIR), National Institutes of Health.  The research agenda of the DIR focuses mostly on immunology and infectious disease research, and flow cytometry is central to many research projects.  The DIR supports approximately 120 principle investigators and 500 postdoctoral and clinical fellows. The Facility currently hosts six high speed cell sorters, five cell analyzers, and one imaging cytometer. Operations covers both BSL2 and BSL3 laboratories.

 

Responsibilities and Duties

  • Contribute to all aspects of facility operation, including flow cytometry analysis and cell sorting with added emphasis on independent operation of cell sorters inside both BSL2 and BSL-3 labs.
  • Perform daily Quality Control and maintenance.
  • Perform troubleshooting of Flow Cytometry equipment.
  • Provide support to researchers in operating analyzers.
  • Assist with user training and basic panel development.
  • Perform sample preparation and staining for flow cytometry analysis.
  • Assist in the preparation of SOPs and related documents for GLP work.
  • Comply with safety guidelines and requirements by following and ensuring strict safety procedures and safety checks.
  • Assist in Research and Development programs for new assays and technologies 
  • Qualifications

    • Positions require bachelor’s or master’s degree in Biology, Immunology, or related field.
  • Minimum five (5) years or more of demonstrated relevant experience in flow cytometry.
  • Experience in the operation of cell sorters is highly preferable.
  • Proficiency with basic laboratory techniques (sample preparation, cell culture) and flow cytometry methods such as immunophenotyping, cell cycle analysis, etc.
  • Experience in high parameter flow cytometry will be an advantage, but training can be provided where required.
  • Good understanding of laboratory biosafety standards.
  • Proficiency in the use of Microsoft Office including knowledge of computer systems and ability to work independently with data analysis programs
  • Ability to multi-task in a busy facility and to approach tasks and obstacles with a team-first mentality.
  • Development of new methods that extend the scope of flow cytometry at the facility will require excellent laboratory skills and a high level of desire to learn new skills


    For additional questions or concerns please contact Stefonie Kelly at stefonie.kelly@axleinfo.com or 202.421.2402.

    if you have a passion for Flow Cytometry and want to join a progressive company, give me a call. We are adding new sponsors and increasing our service offerings to existing sponsors.   Full time roles with benefits. Greg Inguagiato 904-831-5682

    greg.inguagiato@resourcesolutions.com


    Senior Scientist – Flow Cytometry

    Valencia, CA

    R1038817

    Job Summary

    Resource Solutions is working on behalf of our client, Q2 Solutions, the world’s second largest clinical laboratory services provider. Launched in 2015 through joint venture between Quintiles and Quest Diagnostics, Q2 Solutions is focused on helping biopharmaceutical, medical device and diagnostics customers improve human health through innovation that transforms science and data into actionable medical insights.

     

    Responsibilities

    • Assist with optimizing and improving flow cytometry operations
    • Perform routine technical troubleshooting of flow cytometry instruments and assays
    • Perform bench work and routine gating for operational support
    • Performs data analysis and reporting of flow cytometry clinical samples across a variety of cytometry assays.
    • Provides technical expertise, leadership and support for flow cytometry operations for assay/result troubleshooting.
    • Reviews and documents the results of technology transfers and process improvement validations. Collaborates with the Translational Sciences Laboratory where appropriate.
    • Investigate and resolve operational and quality issues within the flow cytometry department.
    • Write and review standard operating procedures and policies for the department.
    • Assist in the qualification and validation of new or repaired instruments.
    • Assists with training for flow cytometry staff for new and existing assays.
    • All responsibilities are essential job functions unless noted as nonessential (N).

     

    Required Knowledge, Skills and Abilities

    • A good understanding of equipment, software and processes of flow cytometry and the overall subject area.
    • Knowledge of clinical and/or research laboratory environment and quality control procedures.
    • Excellent Excel skills.
    • Ability to balance multiple priorities and work to short deadlines.
    • Ability to establish and maintain effective working relationships with co-workers, managers and clients.
    • Good oral communication skills.

     

    Minimum Required Education and Experience

    • Bachelors degree in chemical, physical, biological science, or clinical laboratory science
    • 5+ years of laboratory related experience with Flow Cytometry, experience in regulated clinical lab is preferred
    • CLIA and ASCP certification is highly preferred, not required

     

    About our Client

    With more than 9 million clinical laboratory tests managed annually, we are committed to providing our customers an innovative, progressive and responsive partner with the quality focus, global experience and deep medical expertise integral to drug, medical device and diagnostic development.

    We work collaboratively with our customers, business partners and colleagues to lead the industry and live our customer promise of providing Actionable Insights for Better Health™.

     

    Our commitment to innovation is how we’re building better clinical laboratory services for our customers’ – we call it “the lab of the future.” The lab of the future will help our customers develop drugs faster and more efficiently. Learn more about how we partner with our customers to build the lab of the future through: Tailored Solutions, Delivery Excellence and Shaping Outcomes.

     

    What we Offer

    ·         Major Medical, Vision, Dental, Disability

    ·         Annual bonus

    ·         401(k) with 100% vesting after 2 years

    ·         Tuition Reimbursement up to $6k annually

    ·         Generous paid time off and holiday leave

    ·         Other generous benefits

     

    EEO Minorities/Females/Protected Veterans/Disabled

     

     

     

    Dear All


    Does anyone know if there is a Euroflow users group?


    We are experiencing some issues with EQA material on a certain EQA program. The same issues are not present in our patient material.


    What EQA programs do other people use - especially on Euroflow?

    Any body having issues?


    Almero


    South Africa

    Almero Du Pisani Nov 2 '18 · Tags: euroflow eqa
    we are going to distinguish NKcell population within PBMC through flow cytometry. Our approach is as follows:- 1. From the dot plot of FSC vs SSC we r gating CD-3 Cy5|| CD-56 FITC. 2. To isolate NK cell We are choosing the CD3-/CD56+ cells. 3. As we wish to detect the NK cell dim/bright population, we then gate CD56 FITC||CD16PE population. 4.At this stage we have isolated NK population and also differentiated NK dim/bright cells also. 5. Next we are going to observe CD-328 PE along with CD56 FITC dim/bright. 6. Similarly CD-329 PE will be observed. IMPORTANT NOTE: Our constraints:- 1.we can not perform 6 color facs. 2. We have isotype control having 3 fluorophores namely FITC, PE, Cy5 which can't be altered due to budget constraint. 3.we have access to a BDFacscalibur having 4color only namely FL1,FL2,FL3,FL4. We prefer to perform 2color face. 4. Due to budget constraint , we will set the CD3 and CD16 gates first and then further just gate the PBMC through the previously selected gate. Then we will just tag the PBMC with CD56 & CD328/CD329 is it okay? Waiting for your kind reply.
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