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Cellular measurements by
flow cytometric analysis constitute an important step toward
understanding individual attributes within a population of cells.
Assessing individual cells within a population by protein
expression using fluorescently labeled antibodies and other
fluorescent probes can identify cellular patterns. The technology
for accurately identifying subtle changes in protein expression
within a population of cells using a vast array of technology has
resulted in controversy and questions regarding reproducibility,
which can be explained at least in part by the absence of standard
methods to facilitate comparison of flow cytometric data. The
complexity of technological advancements and the need for
improvements in biological resolution results in the generation of
complex data that demands the use of minimum standards for their
publication. Herein we present a summarized view for the inclusion
of consistent flow cytometric experimental information as
supplemental data. Four major points, experimental and sample
information, data acquisition, analysis, and presentation are
emphasized. Together, these guidelines will facilitate the review
and publication of flow cytometry data that provide an accurate
foundation for ongoing studies with this evolving technology.
D.F. Alvarez et.al. AJP - Lung Physiol February 1,
2010 vol. 298 no. 2 L127-L130
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