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William G. Telford
Curr. Protoc.
Cytom. 59:9.38.1-9.38.12. © 2012 by John
Wiley & Sons, Inc.
Abstract:
This unit describes the use of a novel violet-excited
membrane-binding probe F2N12S [4′-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone]
for the flow cytometric detection of the changes in membrane
asymmetry, fluidity, and charge that accompanies apoptosis. This
reagent inserts into the plasma membrane and undergoes a shift in
emission from orange to green during the membrane alterations
that occur during apoptosis. Since its mechanism of action
differs from annexin V, it can be used in situations where
annexin V binding is problematic, as in adherent cells removed
from their growth substrate. Like annexin V, it can also be
readily combined with other flow cytometric assays for apoptosis,
allowing detailed multiparametric analysis of the apoptotic
process.
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